Final Report to the Research Subcommittee of the Maine Potato Board

 

 

Funded by:                 MTI Cluster Enhancement Award from Maine Technology Institute, Maine Potato Board and Maine Seed Potato Board

 

Project Title:     A New System for Production of Minitubers in the Greenhouse

 

Project Duration: April 1, 2006 to Dec 31, 2006

 

Principal Investigator:  Dr. Vikram Bisht, Porter Seed Potato Farm

 

12 January 2007

 

Final Report for MTI and MPB

 

 

 

EXECUTIVE SUMMARY

 

A small scale trial was conducted in the summer of 2005 to determine the potential of minituber production using nutrient film technique (NFT) and compare productivity against the existing production system using soil-less growing mix.  The trial on the production of minitubers using the NFT system was repeated in 2006.  In 2006, well water was used for the trial in the initial stages, but later changed to reverse osmosis water. The planting for the trial was started in the last week of June and the final harvesting was done by October 31.  Six varieties were planted in NFT system, but many more varieties were planted in grow-mix beds.  Plants were spaced at 4” in the NFT and in the grow-mix beds.  Minitubers upto 7mm size were harvested, data on number and total weight were collected.  Analysis of the data showed that NFT system produced 4 to 8 times more tuber mass and 9 to 24 times more tuber numbers than the regular greenhouse productivity.  It could be inferred from this preliminary trial that NFT system has huge potential to help the Maine seed potato industry.  Delay in planting of trial and high temperature during early growth stage of the seedlings may have prevented the NFT system from reaching its full potential of production.

 

 

 

BACKGROUND

 

The trial of 2005 trial was repeated in 2006 summer. 

 

Minituber production in North America is being done using either the grow-mix beds or the hydroponics systems. The primary hydroponics system being used is the NFT system, as understood from discussions with various potato industry people. However, it is difficult to obtain reliable data on the details of NFT or other production systems. 

 

Many states in the USA and provinces in Canada have fewer (five to six) field generations of potatoes compared to the seven allowed in Maine.  Reduction in the number of field generations would normally improve potato tuber quality in the later generations by reducing exposure to soil-borne diseases and viruses, but it would necessitate a much higher production of early generation seed.  This would require increased production of minitubers. Also, to stay competitive and be able to quickly adopt new varieties, a higher productivity system would be extremely helpful to the Maine potato industry.  Some research has been conducted on such potato production systems in greenhouses, but only a limited amount has been published or made public. It is therefore important to understand the impact of various input factors, which may influence the productivity of the system at our own facility.

 

To stay competitive, Maine potato industry needs to employ innovative means to increase its high quality seed production capability. As done by other agencies, it is essential to keep the details of this technology confidential.

 

 

EXPLANATION OF THE PROJECT:

 

The trial was conducted with the following objectives:

 

a.      Develop a new minituber production system, which would significantly increase minituber production in our greenhouses; and determine if it is cost effective.

b.      Test the system for its adaptability to Porter Farm greenhouse production, and identify points for improvement in the first production cycle to refine it for evaluation in the 2nd year. Use of well water with very low EC (0.1 ms/cm) was tried, to reduce cost of clean water. De-ionized water EC = 0 ms/cm.

c.      Finally, based on the results of this project, set up a commercial scale production system to supply the Maine potato industry with higher volumes of lower cost minitubers and early generation seed.

 

The research trial involved growing potato minitubers in the NFT system and comparing the production to the presently used system of plants in grow-mix beds. 

 

 

1. Systems to compare

 

a.      Soil-less grow-mix medium

Normal mix of peat, Perlite and vermiculite in various proportions – e.g. ProMix, Scotts Mix, often contain wetting agents and some amounts of start-up fertilizer, but regular fertilizer application for the growing crop is also needed. Seedlings were planted at 400 per 4’ square bed at roughly 4” spacing.

 

b.     Nutrient film technique (NFT) system / hydroponics system

This system involves a continuous supply of nutrients through a re-circulation system.  The nutrients are continuously monitored for pH, EC and replenished to maintain uniform nutrient levels.

 

 

Greenhouse Set up: To reduce the cost of water de-ionization, deep well water, with an EC of 0.02 was used.  The greenhouse poly-film cover, which was removed after the 2005 trial, had to be put back on.  To prevent high temperature in the greenhouse (as in 2005), shade cloth was also put on. The sides (4 ft. high from the ground up) and the end walls were covered with screen to allow natural air-flow and still keep it aphid-proof.  The internal floor space of the greenhouse was 30 ft x 48 ft, and was 12 ft. high at the central area.

 

 

Planting:  In May, a large number of seedlings were started for the varieties to be used in the trial so that there would be no shortage of uniform and good-sized plantlets.  In early to mid June, the potato plants were being readied for later planting in the greenhouse. Two NFT systems B1 and B2, with independent re-circulation systems, were set up in mid-June. System B1 had one re-circulation system feeding nutrient solution into 6 separate grow-trays (approx. 2.5 ft x 4 ft); while in system B2 there were no separate trays, but a single large B&W poly-lined bed (approx. 4 ft x 12 ft).  The system was tested for a couple of days to ensure proper running. First planting in the B1 system NFT trays was done on June 19, finishing B1 system planting on June 27.  NFT system B2 was planted on July 6 and 7; two white tuber varieties were separated by red tuber variety, Norland (Table 1 & 2). In total 6 varieties were planted in the 2 systems.  A third NFT system, B3, was set up and planted from late July into early August. This system consisted of ten 10 ft. long trays fed with nutrient solution from a 3rd nutrient re-circulation system. Nine different varieties were planted in this system, using older seedlings (left over from B1 & B2 systems and grow-mix bed plantings).

 

In regular grow-mix beds seedlings were planted at 400 plants/bed or lower, having a plant to plant spacing of about 4”.  Fertilizer application was as per standard protocol used at the farm.

 

The NFT system required daily monitoring and adjustment of the nutrient status, while the grow-mix beds needed daily watering and timely fertilizer application.  Plants in both systems were sprayed with Champion WP fungicide to protect against late blight infection.  Time for various activities in the trial was recorded as best as possible.

 

 

Harvesting: In the NFT system, minitubers were harvested upto 7 times from the same plants, depending upon the NFT system and the variety.  As far as possible, minitubers 15mm or larger were harvested. Harvesting dates for the NFT tubers ranged between Aug 28, and the final harvesting on Oct 31. The grow-mix bed plants were harvested only once, on Oct 12 &13. The comparisons between grow-mix beds and NFT system are made based on average of 20 grow-mix beds and 33 NFT var-system trays. NFT system B3 had poor plant growth, and so data were not utilized for comparisons; but have been presented.  Data were analyzed non-statistically, due to lack of space for replications

 

 

 

Results of the Trial:

 

The nutrient circulation system set-up worked reasonably well.  Temperature records of the screenhouse and the nutrient solution in system B1 showed that the air temperatures reached a peak of 39 oC (102.2oF) on one day; also the nutrient solution temperatures sometimes went above 20 oC (68oF) (which is considered to be above the optimal temperature). Below are the monthly high and low for the air and nutrient temperatures:

 

 

Air Temp

Low (Morning)

Nutrient Temp Low (Morning)

Air Temp High (Afternoon)

Nutrient Temp High (Afternoon)

June

16

14

38

24.5

July

19.3

16.2

39

26.6

Aug

9.8

10.7

31

19.3

Sep

0.8

4.6

28.7

20.8

Oct

-2.0

3.1

28.2

19.6

 

A liquid cooling system was used in the NFT systems to buffer the nutrient solution temperature. However, the cooling system’s capacity was seriously challenged at very high and very low temperatures.  Below 10C and over 20C, the nutrient solution temperature affects plant growth and tuber production.

 

Screenhouse set-up worked well for the NFT trial (photos).

Early in the trial, the plants showed signs of nutrient imbalance, and could have been due to the well water being used.  The nutrient solution had to be dumped a few times, and de-ionized water was used to make the nutrient solution. The plants recovered from the signs of nutrient imbalance.  Reverse osmosis water was later used to make up for the regular water losses due to plant uptake and transpiration.

 

As in 2005, in 2006 also, system B2 produced much more than B1, even though B2 planted later- the average weight of MTs potentially produced by 400 plants in B2 (142 lbs) was about 80% times higher than from B1 (79 lbs), and number of MTs was 20% higher.  Within each NFT system, there was significant variation between varieties, the number of MTs produced per plant ranged from low of nearly 11.6 to about 39.1 (Table 1).  Production in B3 system was extremely poor and may be due to algae growth in the system and use of older seedlings. The plants grew very poorly and only one or two harvestings were possible.

 

Average production of 8.56 lbs per grow-mix bed with 400 plants is close to what has been normally been observed in our greenhouses. This production would have been higher, but for the poor performance of Norwis, RB and Green Mountain varieties. No specific reason could be ascribed to their poor production.

 

Productivity per plant of minitubers in the NFT systems (Bench 1 and Bench 2) was significantly higher than the grow-mix beds (Tables 1, 2 & 3). Number of MTs in the NFT system were 14.2 times more than the grow-mix beds, while the mass of MTs produced was 12 times higher.   The overall picture of the two systems shows that NFT produced 26. MTs/plant compared to 1.81 in the grow-mix beds.

 

 

 

Total

Plants Used

Total MTs

MTs/plant

 

2005

NFT  (Both Benches)

409

10,291

25.16

15.9

 

Grow-Mix Beds

7817

12,323

1.58

1

2006

NFT (2 systems)

306

8869

26

14.2

 

Grow-Mix beds

10485

19001

1.81

1

 

 

Another way to compare the data was comparison of production by 400 plants in the two systems in 2006:

 

Production System

Minitubers from 400 plants

Number

Weight (Lbs)

NFT

10393

100.1

GrowMix Beds

759

8.56

NFT : GrowMix Beds

13.7 : 1

11.7 : 1

 

 

In 2006 trial, there was increased automation for nutrient management, however, continuous monitoring was essential for recording data. Due to experience gained in the first year and also due to increase in automation, less time was spent on the 2 older re-circulation systems; however, the 3rd system of ten 10 ft. long trays took more time than expected. Also this system did not perform upto expectation. This may partly due to algae growth and older seedlings. This system would be improved to prevent algae growth and younger seedlings will be used.  

 

 

All funds for the research grant were received in a very timely manner

           

 

 Granting Agency

Award

Aug 01

Maine Technology Institute

$ 7,250

Sep 27

Maine Potato Board

$ 5,000

Allocated, in-kind

Maine Seed Potato Board

$ 5,500

 

 

$17,750.00

 

 

 

 

Screenhouse set-up worked well for the NFT trial (photos included).


Conclusions:

 

In the NFT system, a significantly higher number and also higher mass of minitubers were produced. Minitubers from NFT system were harvested when they reached the desired size; uniform size of minitubers is helpful during planting and leads to uniform emergence.

 

Production in system B3 was poor due to late planting and growth of algae in the system; this will be rectified in 2007 production.  Deep well water at the farm, in-spite of having very low EC, is not good enough for NFT production system. Reverse Osmosis water will be used for future production; and this may be a cheaper option than de-ionized water.

 

The fact that the number of minitubers produced per plant in NFT was significantly higher than in grow-mix beds, there is a good potential for using this technology at the Porter Seed Potato Farm. Adopting this technology at the Farm would be cost effective due to fewer plantlets needed for production and also savings on time and labor costs for tissue culture too.  Another critical benefit of using this system would be rapid increase of early generation seed, when the industry needs the change; e.g. change in preference of clones of Russet Burbank, Red La Soda and Snowden.

 

To exploit the full potential of the NFT system for minituber production it would be important to set a full-scale commercial system. Help from Maine’s potato industry would be needed to achieve the goal.